Cat rhinotracheitis, calicivirus disease, and panthenopia are common diseases in cats, which bring great pain to pets and cause great trouble to owners; the infection rate of these three viruses is above 30%, and it has become the three most important viral infectious diseases that endanger the health of pet cats. It is very harmful to cats and is prone to mixed infections. Prevention and control are particularly important. Therefore, the cat triple vaccine is basically a must-choose immune breed for pet cats.
Triple inactivated vaccine is a safe and effective vaccine to prevent cat rhintracheitis, calicivirus disease, and panthenopia. Vaccination can help cats build immunity and reduce the risk of infection. This vaccine not only protects cats' health, but also protects humans from infection.
In 2022, my country's pet vaccine market size was 7.6 billion yuan, an increase of 17% year-on-year. Driven by the continued prosperity of the "cat economy", the cat vaccine market size reached 3.4 billion in 2022, an increase of 30.1% year-on-year, accounting for 45.5%; among which, the cat triple vaccine market can reach 2.78 billion yuan, accounting for 80.7% of the cat vaccine market.
cat triple vaccine purification process
inactivated vaccine is a type of vaccine that is inactivated by inculturing viruses or bacteria and is subjected to physical or chemical treatment to obtain no infectious activity. Compared with attenuated vaccines and genetically engineered vaccines, inactivated vaccines have the advantages of short R&D cycle, relatively mature preparation process, no infection virility and high safety. The production process of inactivated vaccines mainly includes five steps: culture of pathogens, inactivation of pathogens, extraction and purification of pathogens, formulation and packaging of vaccines, and quality control of vaccines. This article mainly introduces the downstream purification scheme of cat triple vaccine.
After the virus harvest solution was concentrated by ultrafiltration, the special medium for Virus purification of BoJin Bio was solved by flow-through mode or by large-pore GPQ-60 (1000) strong anion exchange medium through binding to elution mode. The amount of supernatant was large in a single batch, and the purity of impurity SDS-page after purification was less than 5%, and the virus yield and titer effect was low. Part of the data of the
experiment:
Bojin Biosciences selected the corresponding composite mode medium with the interception range of 400kDa and 700kDa for experiments on three viruses FPV (diameter 20-30nm), FCV (diameter 20-30nm) and FHV (diameter 100nm).
The final removal effect and its impact on virus yield:
Judging from the experimental results, for the two virus FPV and FCV in diameter 20-30nm, the MixQ400S of Bojin Bio has better removal effect, and the virus FHV of about 100nm and MixQ400S/700S have better removal effect; the special media for virus purification of Bojin Bio has less impact on virus yield in the flow-through mode.